Alloxaninduced diabetes, a common model for evaluating. It is better to standardize it first by jain et al. Mice administered a single intraperitoneal injection of alloxan monohydrate at a dose of 150 mgkg showed progression of typei diabetes symptoms figure 2. Alloxan induced diabetic rabbits as animal model for diabetes. Histopathological abnormalities of prolonged alloxaninduced diabetes mellitus in rabbits, international. The first group 5 rabbits was used as control and fed with standard chow and tap water. Effects of aqueous extract of alfalfa on hyperglycemia and. Research article alloxan induced diabetes mellitus in.
The present paper is an extension of this study of the mechanism of alloxan hypoglycemia. Alloxan dose of 120, 140, 160 and 180 mg kg 1 body weight was given intraperitonealy to experimental rats in groups of 6 animals for each dose. Experimental model of induction of diabetes mellitus in rats. Study the effect of phytoesterol of ceratoina female rabbits.
Pdf pathomorphological effects of alloxan induced acute. On microscopic study these observers found necrosis of all the islets of langerhans while the. If the inline pdf is not rendering correctly, you can download the pdf file here. In 1937 jacobs 1 found that alloxan 2 the ureide of mesoxalic acid produced fatal hypoglycemia when injected intravenously into rabbits. Altered glucose metabolism and hypoxic response in alloxan. The alloxan model of diabetes was first described in rabbits by dunn, sheehan and mcletchie in 1943. Antidiabetic effect of ipomoea batatas in normal and. Antidiabetic activity of bauhinia forficata extracts in. This study undertaken to investigate the was beneficial effect of vitamin c and zinc on some biochemical parameter when induced diabetic in rabbits by alloxanrabbits groups. Streptozotocin induced acute clinical effects in rabbits. In the alloxan induced diabetic rats showed that extract at 500 mgkg decreased significantly fasting plasma glucose level by 61. Diabetic atherosclerosis was induced in rabbit iliofemoral arteries by injecting alloxan 100 mgkg, injuring the arteries using a balloon, and feeding with a 0.
It has been successfully induced in a variety of animal species. The effect of the injection of diabetogenic doses of alloxan on blood sugar levels. In remaining ten rabbits, diabetes mellitus was induced by alloxan monohydrate 150 mgkg by intraperitoneal administration. Since then, alloxan diabetes has been commonly utilized as an animal model of insulindependent diabetes mellitus iddm. A total of 90 male and female japanese rabbits weighing 1. Mar 23, 2012 diabetes was induced by streptozotocin or alloxan injection. Since oxidative stress is mediated by hyperglycemiainduced generation of free radical, it is supposed that compounds with hypoglycemic and antioxidative properties would be useful antidiabetic agents.
Blood samples 10ml were collected from each animals at end of experiment by the heart cardiac puncture. Alloxaninduced rats,antidiabetes, diabetes, ipomoea batatas, phytochemical analysis. The anhydrous derivative ocnhco 2 co is also known as well as a dimeric derivative. Alloxan diabetes and streptozotocin diabetes figure 1 shows a schematic diagram of the tetraphasic and triphasic blood glucose responses induced by alloxan and streptozotocin, respectively, when injected 22.
Antidiabetic effect of ipomoea batatas in normal and alloxan. Metformin was orally administered at 50 mgkg, while the extract was administered both to normal and diabetic rabbits at 100, 200 and 300 mgkg. When they finish that diet, ordinary food was given to those. Similar experiments conducted by dunn, sheehan and mcletchie 3 confirmed the hypoglycemic action of alloxan. The responses are accompanied by corresponding inverse changes in. Diabetes mellitus, rabbits, alloxan abstract diabetes mellitus dm a disease which is characterized by hyperglycemia, lipoprotein abnormalities and oxidative stress. Pdf in present study hypoglycaemic effects of the crude powdered c.
Changes in blood glucose f profile of alloxaninduced diabetic rabbits for 26. Dec 18, 2007 alloxan has two distinct pathological effects. The present study was carried out to evaluate the antidiabetic activity of vinca rosea methanolic whole plant extracts in alloxan induced diabetic rats for 14 days. As seen from the data in figure 2, fasting blood glucose levels were reduced versus vehicle treatment following two weeks of oral treatment with glyburide 39.
The mechanisms of alloxan and streptozotocininduced diabetes. On microscopic study these observers found necrosis of. The present study investigated metabolic changes and their relationship to vascular histopathological changes in the atherosclerotic arteries of rabbits with alloxaninduced diabetes. Xanthine oxidase inhibitor allopurinol prevents oxidative. Diabetes mellitus was induced by a 120 mgkg body weight intravenous injection of alloxan.
Alloxan exerts its diabetogenic action when it is administered parenterally. Alloxan and streptozotocin are toxic glucose analogues that preferentially accumulate in pancreatic beta cells via the glut2 glucose transporter. Antidiabetic activity of vinca rosea extracts in alloxan. The responses are accompanied by corresponding inverse changes in plasma insulin and sequential ultrastructural. Essential oils samples were obtained from the aerial parts of the plant by hydrodistillation and analyzed by gcms. This study was to create a longterm rabbit model of diabetes mellitus for medical studies of up to one year or longer and to evaluate the effects of chronic hyperglycemia on damage of major organs. The mechanisms of alloxan and streptozotocininduced. Attenuation of oxidative damage in alloxan induced diabetic.
These are some of the earliest known organic compounds. Alloxaninduced diabetes mellitus how is alloxaninduced. Alloxaninduced and insulinresistant diabetes mellitus. The effect of aepe leaf extract on blood glucose, insulin, hemoglobin and. May 24, 2014 mice administered a single intraperitoneal injection of alloxan monohydrate at a dose of 150 mgkg showed progression of typei diabetes symptoms figure 2. Alloxan has been widely used to induce experimental dm in animals such as rabbits, mice and dogs with different grades of disease severity by varying the dose of alloxan used 8 causing alloxan. Histopathological abnormalities of prolonged alloxaninduced. Alloxan monohydrate is a common drug used for developing experimental diabetes in animals, as streptozotocin, though less toxic than alloxan is considerably costlier. The present study described the phytochemical profile of lavandula stoechas essential oils, collected in the area of aindraham northwest of tunisia, as well as their protective effects against alloxaninduced diabetes and oxidative stress in rat. The name is derived from allantoin, a product of uric acid excreted by the fetus into the allantois, and oxaluric acid derived from oxalic acid and urea, found in urine. A single dose of alloxan monohydrate 100 mgkg was given intravenously to 20 young new zealand white rabbits. Alloxan is one of the frequently used betacytotoxic agents for the induction of type1 diabetes mellitus in animal models and is the drug of choice in rabbits. In animals, it can be induced by partial pancreatectomy or by the administration of diabetogenic drugs such as alloxan, streptozotocin, ditizona and antiinsulin serum. Blood glucose lowering activity of aloe based composition.
Alloxaninduced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. They also exhibit a variety of biological activities. Extracts were administered daily for 7d at doses of 200 and 400mgkg, p. In vivo penile erection length induced by drugs was measured in conscious rabbits. K p v o o evaluation and optimization of effectivedose. The evaluation of soybean extracts in alloxan induced diabetic rabbits. Alloxan induced and insulinresistant diabetes mellitus affect semen parameters and impair spermatogenesis in male rats 108 african journal of reproductive health african journal of reproductive health vol. Alloxan induced diabetic rabbits as animal model for. Effect of trigonella foenum methi on fasting blood glucose levels in.
Erectile properties of the rhokinase inhibitor sar407899 in. Bailey cc, bailey ot, leech rs 1946 diabetes mellitus in rabbits. Sar407899 dosedependently relaxed the precontracted corpora cavernosa in all species, with similar potency and efficacy in healthy vs diabetic rats, wky vs shr rats, healthy vs diabetic rabbits. Alloxan, diabetes mellitus, long evans rat, induced diabetes, toxicity. Streptozotocin stz is used for induction of type1 diabetes mellitus in animal models. The mechanism of alloxan and streptozotocin action in b.
The mechanism of alloxan and streptozotocin action in b cells. Alloxan has been administered in single or multiple doses, through different routes intraperitoneal. Similarly, alazzawie and alhamdani 19 reported that dietary oleuropein in alloxan diabetic rabbits reduced plasma mda levels to the level of nondiabetic animals. Diabetes mellitus were induced in twenty four starved pregnant female rabbits by giving alloxan injected by one ml size syringe and in dose 150mg\kg for three days. Its betacytotoxic action results in sudden release of insulin leading to. Antidiabetic activity of aqueous extract of leptospermum. The severe diabetes produced by alloxan results in blood sugar levels equivalent to a total pancreatectomy, hence sulphonylureas such. We used alloxan to induce diabetes in rats and rabbits for screening the antidiabetic activity of test compounds. Attenuation of oxidative damage in alloxan induced. These agents selectively destroy the langerhans islet. Alloxan monohydrate is used to induce diabetes mellitus in albino rats in the dose of 120mgkg i. Effect of trigonelline and ethanol extract of iraqi fenugreek. Modulation of hematological indices of normal and alloxan. Nov 29, 2015 models for insulin dependent diabetes mellitus iddm alloxan induced diabetes principlemechanism interacts with sulfhydryl groups and inhibit it following its uptake by the beta cells, alloxan interacts with sulfhydryl containing cellular components, particularly enzymes known to be essential for beta cell function.
The best known druginduced diabetes model is the alloxan diabetes. Research article alloxan induced diabetes mellitus in wistar. Schuh wholeplant in normal and alloxaninduced diabetic rabbits. Diabetes is a disease that constitutes multiple sources of free radicals, thus oxidative stress is expected to have a double impact. Hypoglycaemic effect was comparable to that of glibenclamide. Alloxan and streptozotocin are toxic glucose analogues that preferentially. Alloxan induced diabetes is a form of insulindependent diabetes mellitus that occurs as a result of alloxan administration or injection to animals. Comparative toxicity of alloxan, nalkylalloxans and ninhydrin to isolated pancreatic islets in vitro.
Another 12 agematched normal rabbits were used as controls. The present study was conducted to investigate antidiabetic efficacy of extracts of nigella sativa linn. Effect of trigonelline and ethanol extract of iraqi. Hypoglycemic and antioxidant effect of oleuropein in alloxan. This study aimed to create diabetic animal models and to provide an exact and detailed account of alloxan induced diabetes in rabbits. They were randomly and equally divided into the control group c, n30, alloxaninduced group with dm dm, n30 and allopurinoltreated group with dm allo, n30. The results showed a significant improvement in the stress induced oxidation parameters by alloxaninduced diabetes in rabbits. The methanolic whole plant extract at high dose 500 mgkg exhibited significant antihyperglycemic activity than whole plant extract at low dose 300 mgkg in diabetic rats. The optimum effective dose of alloxan was found to be 140 mgkg bw for induction of stable diabetes in long evan rats. Alloxan induced rats,anti diabetes, diabetes, ipomoea batatas, phytochemical analysis.
The ethanol extract of fenugreek seeds significantly decreases the plasma malondialdehyde mda from 2. Sir, we read with interest the article titled anomalies in alloxaninduced diabetic model. The effect, however, was more pronounced in diabetic animals in which administration for 4 weeks after alloxan induced diabetes, significantly reduced blood glucose levels. Hypoglycemic and antioxidant effect of oleuropein in alloxandiabetic rabbits article in life sciences 7812.
Alloxan induced diabetic rabbits n 28 were divided. Alloxan, sometimes referred to as alloxan hydrate, refers to the organic compound with the formula ocnhco 2 coh 2. The present study investigated metabolic changes and their relationship to vascular histopathological changes in the atherosclerotic arteries of rabbits with alloxan induced diabetes. The pdf file you selected should load here if your web browser has a pdf. Introduction diabetes mellitus diabetes is a group of metabolic diseases in which a person has high blood sugar, either because the body does not produce enough insulin, or because cells do not respond to the insulin that is.