The glomax 2020 luminometer combines instrumentation and software in a complete solution that includes bioluminescent assays, protocols and support. Biorad gel doz ez with image lab software dnaprotein gels and blots useroperated. This plate reading luminometer is optimized with for use with promega cell and gene reporter assays and comes with over 30 preloaded assay protocols. Kitchen design software bathroom design software office interior design software furniture manufacturing software retailer interior design. Larvae infected with hetct ijs exhibited an average bioluminescence of 6. The glomax 20 20 luminometer combines instrumentation and software in a complete solution that includes bioluminescent assays, protocols and support. Activity was normalized relative to levels of renilla luciferase expressed by the internally cotransfected constitutive control. Neu1 sialidase controls gene expression and secretion of il6. Functional analysis of snps in the ercc5 promoter in. Naturally existing isoforms of mir222 have distinct. Select spreadsheet interface software on the list and click the addremove button. Upgrade your system later as your laboratory needs change.
In addition to high performance, the glomax 2020 blends userfriendly. Dlready validated luminometers the singletube and plate luminometers that have passed the dlready validation criteria are listed below. Glomax navigator microplate luminometer luminescence. An in vitro and in silico investigation of human pregnane x. Breluc2p hek293 cell line is a clonal derivative of human embryonic kidney 293 hek293 cells. It gathers 26 collaborators, including two professors of university, 15 assistant professors and lecturers and nine engineers and technicians. Luciferase activity was measured using the dualluciferase reporter assay promega and a glomax 2020 luminometer promega as described previously mudo et al. This multimode plate reader offers advanced detection options in a single, easytouse instrument.
An in vitro and in silico investigation of human pregnane. Allow at least 6 inches 16cm of vertical clearance to open and close the lid. Promega 2020 singletube luminometer useroperated nanodrop nd and 2000c spectrophotometers useroperated qubit 2. Interface tubing and valves for auto injector system.
Home 2020 design new zealand 2d 3d kitchen, bathroom. The glomax 2020 singletube luminometer is popular with labs that have low throughput needs. Global customer support technical service scientists knowledgeable sales force training and troubleshooting reagents and instruments new protocol implementation. After 48 h of transfection, the cells with different treatments were lysed with buffer of the dual luciferase detection kit e1910, promega corporation, madison, wi, usa. Glomacs leadingedge training courses provide business professionals the skills they need to develop their competency, improve their performance, and drive business success.
The 2020 program is a great program but it doesnt do the designing for you. Cells were harvested 72 h posttransfection and assessed using a glomax 2020 luminometer promega. Place the glomax 2020 luminometer on a flat, level surface. Neu1 sialidase controls gene expression and secretion of. Although there are some sporadic reports which demonstrate the importance of each mirna in this cluster in breast cancer, the biological roles of this cluster as a whole and its regulation mechanisms in breast cancer are still unclear. In addition to high performance, the glomax 2020 blends userfriendly operation and a small footprint with flexible purchasing options. After 24 h, psicheck2 vectors nm were cotransfected with 10 nm mirna mimics or negative control rna, using lipofectamine 2000 invitrogen following the manufacturers instructions. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. Data were processed and analyzed by the analysis of variance anova and the bonferroni posttest or unpaired tests using graphpad prism 6. Early viral entry assays for the identification and. Luciferase activity was measured using the glomax 2020 luminometer fluorescence detector promega corporation, madison, wi, usa. Production of pseudotyped particles to study highly. Dji phantom 4 footage glomax solutions llc duration.
Cells were incubated for 2 days in the differentiation medium and 50 ngml fgf21 was added for an additional 24 h. To reserve time on the gel doc go to our iigb reservation and billing site applications. Thus, the hpxr agonistic activity of seven additional pfcs was investigated. The result of this design is an ultrasensitive instrument with superior performance that is easy to use, affordable, and can be customized to your labs needs.
Our talented team of international instructors have exceptional credentials complemented by practical, realworld experience. Highperformance microplate reader for detecting luminescence, fluorescence and absorbance. Whether youre looking for training, a custom catalogue virtual library. Evaluating bacterial growth using the bactiterglo assay. The agonistic hpxr activity of 11 pfcs, which was published by zhang et al. Luciferase activity was determined after a 24h incubation using the dualluciferase reporter assay system promega by glomax 2020 luminometer promega. Applitek ezatp online analyzer automated microbial.
The homogeneous assay involves adding a single reagent bactiterglo reagent directly to bacterial cells cultured in medium and measuring luminescence. Biorad gel doc ez with image lab software dnaprotein gels and blots useroperated for pricing see our rates page. Micrornas can generate thresholds in target gene expression. The glomax 2020 reads one sample at a time and can use microfuge tubes, 12 x 50 mm tubes or 35 mm petri dishes as the input vessel. Glomax 2020 quantifluor p quantifluor st price 2011, promega corporation. For more information on the luminometer of interest, select from the appropriate company web pages provided below. Choose expert led short courses, trainings, seminars, workshops, inhouse training and.
Become a 2020 design expert with classroom training that gets you out of the office and allows you to concentrate in a dedicated learning. Connect the power supply into the back of the luminometer. Glomax discover, explorer, navigator, and 2020 luminometer offer a range of detection options. Transcriptional regulation, coupled to the expression of a luciferase reporter gene, is regularly used to study a wide range of biological events in. Luciferase detection instrumentation guide thermo fisher. The glomax explorer system is a highperformance microplate reader developed with promega fluorescent and luminescent reagents to provide a simple means of detecting advanced chemistries. By standard cell culture techniques, obtain an 8090% confluent 75 cm 2 flask of hek293t17 cells passaged in complete dulbeccos modified eagles medium dmemc containing 10% volvol fetal bovine serum fbs, 10 mm 42hydroxyethyl1piperazineethanesulfonic acid hepes, 100 iuml. The antipsychotic agent flupentixol is a new pi3k inhibitor and potential anticancer drug for lung cancer. Glomax navigator is an easytouse microplate luminometer with superior luminescence detection capabilities.
The department of oenology and chemistry was founded in february 2019. Furthermore, sample pellets were reconstituted with 1x pbs and used to determine the protein concentrations by using the bca assay kit from thermo fisher. They are not for the turner biosystems 2020n singletube luminometer or promega glomax 2020 luminometer. The welch 8890a, direct drive vacuum dual stage rotary vane mechanical vacuum pump offers an excellent ultimate pressure of 1 x 101 torr. This instrument is used to image nucleic acid and protein gels.
A total of 170 patients who were cytologically or histologically confirmed with advanced colorectal cancer crc, at least 1 measurable lesion, and underwent oxaliplatinbased chemotherapy were studied. Downloadable software and firmware for promega instruments, including luminometers, fluorometers and dna. It allows the user to check the reproducibility, sensitivity, and linearity of the luminometer. After incubation, two cadavers from each plate were measured with a glomax 2020 luminometer promega, wi, usa. The glomax20 20 luminometer also features optional modules for measuring certain fluorescent materials including fluorescein, hoescht dye, 4mu, picogreendye and ribogreen dye. In addition to high performance, the glomax 20 20 blends userfriendly operation and a small footprint with flexible purchasing options. These accessories are only for the nowdiscontinued turner biosystems luminometer model td 2020. Activity was normalized relative to levels of renilla luciferase expressed by the internally cotransfected constitutive control vector, prltk promega. The glomax 2020 luminometer in combination with the dualluciferase reporter dlr assay system provides a convenient, rapid and sensitive procedure for quantifying gene expression. Anatomy of a fighter channel subscribe subscribed unsubscribe. For use with discontinued glomax 96 microplate luminometer instrument. In addition, a lownoise photomultiplier tube ensures that collected light is not compromised.
The bactiterglo microbial cell viability assay provides a method for determining the number of viable microbial cells in culture based on quantitation of the atp present. We therefore gathered salient information about many of the instruments available to date. Applitek ezatp online analyzer automated microbial water. To achieve this plugandplay usability, the glomax 2020 combines a color touch screen with an intuitive user interface. Cl curves were obtained in a glomax luminometer td 2020, turner designs, and the results are expressed in nm of trolox. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. To fit your specific needs and improve the quality of your service. B transcription factor, which is responsible for regulating. Position the luminometer so that the touch screen faces you. Promega glomax detection systems flexible solutions for. These cells contain a luciferase gene luc2p under the control of a minimal tata promoter with multiple nuclear factor.
The glomax 2020 luminometer is designed to provide ultrahigh performance for bioluminescent and chemiluminescent assays. Get the best deals on luminometers when you shop the largest online selection at. The mir18396182 cluster is a conserved polycistronic microrna mirna cluster which is highly expressed in most breast cancers. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. The 18 pfcs were used as a training set to develop the classic and 3d qsar models.
These pumps are commonly used for vacuum furnace evacuation, backing turbomolecular pumps, backing diffusion pumps, rotary evaporation. When you receive your luminometer, please inspect the package carefully to make sure all accessories are present. Differences in cnosinos activity during resistance to. Samples were taken at various time points, and the bactiterglo assay was performed.